Succinyl-CoA Synthetase Activity Assay Kit (Colorimetric) (ab196989)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Purified mitochondria, Suspension cells, Tissue
- Sensitivity: 100 µU
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Developer 1 vial NADH Standard Yellow 1 vial Positive Control 1 vial SCS Assay Buffer 1 x 25ml SCS Enzyme Mix 1 vial SCS Substrate Mix 1 vial -
Research areas
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Function
Catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA. -
Tissue specificity
Widely expressed. Not expressed in liver and lung. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. -
Involvement in disease
Mitochondrial DNA depletion syndrome 5 -
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Alternative names
- A BETA
- A SCS
- ATP specific succinyl CoA synthetase subunit beta
see all
Images
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NADH Standard curve
Typical NADH Standard Curve using colorimetric reading.
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SCS activity curve in a variety of samplesSuccinyl-CoA Synthetase activity measured in mitochondria isolated from bovine heart, yeast (P. pastoris) and MCF-7 cells and in Jurkat cell lysate. The graph also shows data from the SCS Positive Control included in the kit and Blank background baseline.
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SCS activity in a variety of samplesSuccinyl-CoA Synthetase specific activity in mitochondria prepared from yeast (P. pastoris, 1.14 μg), bovine heart (1.1 μg) and MCF-7cells (0.5 μg), and in Jurkat cell lysate (25 μg).