Anti-NF-kB p65 antibody (ab140751)
Key features and details
- Chicken polyclonal to NF-kB p65
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgY
Overview
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Product name
Anti-NF-kB p65 antibody
See all NF-kB p65 primary antibodies -
Description
Chicken polyclonal to NF-kB p65 -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human NF-kB p65 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
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Positive control
- This antibody gave a positive signal in the following lysates: HeLa whole cell; Jurkat whole cell; Rat spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA -
Concentration information loading... -
Purity
Protein L purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-NF-kB p65 antibody (ab140751)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: NF-κB p60 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab140751 observed at 70 kDa. Red - ab8245 loading control, observed at 37 kDa.
ab140751 was shown to specifically react with NF-κB p65 in wild-type HAP1 cells. No band was observed when NF-κB p65 knockout samples were used. Wild-type and NF-κB p65 knockout samples were subjected to SDS-PAGE. ab140751 (NF-κB p65) and ab8245 (loading control to GAPDH) were diluted 1 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with IRDye® 800CW Donkey anti-Chicken IgG (H + L) and Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216779 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
Western blot - Anti-NF-kB p65 antibody (ab140751)All lanes : Anti-NF-kB p65 antibody (ab140751) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Spleen (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
