Human Serpin B2 ELISA Kit (PAI-2) (ab272480)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 110 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Serpin B2 ELISA Kit (PAI-2)
See all SerpinB2/PAI-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Protein 8 2.8% Inter-assay Sample n Mean SD CV% Protein 3 4.24% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
110 pg/ml -
Range
312.5 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 84 80% - 90% Cell Lysate 85 85% - 85% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Serpin B2 ELISA kit (ab272480) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Serpin B2 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Serpin B2 with 110 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Serpin B2, also known as plasminogen activator inhibitor-2 (PAI-2), is a protein that belongs to the serpin family of protease inhibitors. Serpin B2 exists in two forms, a 47kDa immature form and glycolsylated 60 kDa mature. The majority of clade B (ov-serpins) of the serpin family inhibit serine and/or papain-like cysteine proteinases and protect cells from exogenous and endogenous proteinase-mediated injury. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. Serpin B2 shares 76% and 73% sequence homology with mouse and rat, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Serpin B2 (PAI-2) Capture Antibody 1 x 600µl 10X Human Serpin B2 (PAI-2) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Serpin B2 (PAI-2) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Cytoplasm. Secreted > extracellular space. - Information by UniProt
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Alternative names
- HsT1201
- Monocyte Arg serpin
- Monocyte Arg-serpin
see all -
Database links
- Entrez Gene: 5055 Human
- Omim: 173390 Human
- SwissProt: P05120 Human
- Unigene: 594481 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Serpin B2 standard curve in 1X Cell Extraction Buffer PTR.The Serpin B2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Serpin B2 in human A431 cellsbased on a 200 μg/mL extract load and placenta tissue extract based on a 30 μg/mL extract load. The concentrations of Serpin B2 were measured in duplicate and interpolated from the Serpin B2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin B2 concentration was determined to be 18.93 ng/mL in A431 extract and 18.79 ng/mL in placenta extract.
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Sandwich ELISA - Human Serpin B2 ELISA Kit (PAI-2) (ab272480)To learn more about the advantages of recombinant antibodies see here.
