Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Chromogranin C/SGII antibody (ab104367)

Key features and details

  • Rabbit polyclonal to Chromogranin C/SGII
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Chromogranin C/SGII antibody
    See all Chromogranin C/SGII primary antibodies

  • Description

    Rabbit polyclonal to Chromogranin C/SGII

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Chromogranin C/SGII aa 400-500 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab115938)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Kidney; Human Lung; Human Adrenal gland; Human Liver.

  • General notes

    Previously labelled as Chromogranin C

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab104367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Monkey
Macaque monkey
Orangutan
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides.

  • Sequence similarities

    Belongs to the chromogranin/secretogranin protein family.

  • Cellular localization

    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt

  • Database links

    • Alternative names

      • CHGC antibody
      • Chromogranin C antibody
      • Chromogranin-C antibody
      • EM66 antibody
      • SCG2 antibody
      • SCG2_HUMAN antibody
      • Secretogranin II antibody
      • Secretogranin-2 antibody
      • Secretoneurin antibody
      • SGII antibody
      • SN antibody
      see all

    Images

    • Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml

      Lane 1 : Human kidney tissue lysate - total protein (ab30203)
      Lane 2 : Lung (Human) Tissue Lysate (ab4014)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 64 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 90 seconds


      The band observed at 64 kDa could potentially be a cleaved form of Chromogranin C due to the presence of a 27 amino acid signal peptide and 3 amino acid propeptide.
    • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody (ab104367)
      Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody (ab104367)

      ICC/IF image of ab104367 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104367 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml

      Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 64 kDa why is the actual band size different from the predicted?
      Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    References (0)

    Publishing research using ab104367? Please let us know so that we can cite the reference in this datasheet.

    ab104367 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml

      Lane 1 : Human kidney tissue lysate - total protein (ab30203)
      Lane 2 : Lung (Human) Tissue Lysate (ab4014)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 64 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 90 seconds


      The band observed at 64 kDa could potentially be a cleaved form of Chromogranin C due to the presence of a 27 amino acid signal peptide and 3 amino acid propeptide.
    • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody (ab104367)
      Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody (ab104367)

      ICC/IF image of ab104367 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104367 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      Western blot - Anti-Chromogranin C/SGII antibody (ab104367)
      All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml

      Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 64 kDa why is the actual band size different from the predicted?
      Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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