Anti-Chromogranin C/SGII antibody (ab104367)
Key features and details
- Rabbit polyclonal to Chromogranin C/SGII
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chromogranin C/SGII antibody
See all Chromogranin C/SGII primary antibodies -
Description
Rabbit polyclonal to Chromogranin C/SGII -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Chromogranin C/SGII aa 400-500 conjugated to keyhole limpet haemocyanin.
(Peptide available asab115938) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Kidney; Human Lung; Human Adrenal gland; Human Liver.
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General notes
Previously labelled as Chromogranin C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MonkeyMacaque monkeyOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 7857 Human
- Entrez Gene: 100174732 Orangutan
- Omim: 118930 Human
- SwissProt: P13521 Human
- SwissProt: Q5R4M6 Orangutan
- Unigene: 516726 Human
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Alternative names
- CHGC antibody
- Chromogranin C antibody
- Chromogranin-C antibody
see all
Images
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Western blot - Anti-Chromogranin C/SGII antibody (ab104367)All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate (ab4014)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band observed at 64 kDa could potentially be a cleaved form of Chromogranin C due to the presence of a 27 amino acid signal peptide and 3 amino acid propeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody (ab104367)ICC/IF image of ab104367 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104367 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Chromogranin C/SGII antibody (ab104367)All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml
Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
Datasheets and documents
References (0)
ab104367 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Chromogranin C/SGII antibody (ab104367)All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate (ab4014)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band observed at 64 kDa could potentially be a cleaved form of Chromogranin C due to the presence of a 27 amino acid signal peptide and 3 amino acid propeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody (ab104367)
ICC/IF image of ab104367 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104367 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Chromogranin C/SGII antibody (ab104367)All lanes : Anti-Chromogranin C/SGII antibody (ab104367) at 1 µg/ml
Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
