Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
Suitable for: WB, IP, IHC-P, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-CDK5RAP3 antibody [EPR11294(B)]
See all CDK5RAP3 primary antibodies
Description

Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
Host species

Rabbit
Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human CDK5RAP3 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q96JB5
Positive control
- WB: Jurkat, HeLa, A431, CTLL-2, EL4, H9 and 293T cell lysates. IHC-P: Human pancreas and stomach tissue. Flow Cyt: HEK-293T cells. IP: CDK5RAP3 IP in HEK-293T cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11294(B)
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Apoptosis Markers
- NFkB
- RelA (p65)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/2000 dilution (Purified)

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 57 kDa
Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (Purified)

Lane 1 : CTLL-2 (Mouse T lymphocyte) whole cell lysates
Lane 2 : EL4 (Mouse lymphoma T lymphocyte) whole cell lysates
Lane 3 : H9 (Human lymphoma T lymphocyte) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 57 kDa
Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab157203 & HEK-293 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.
Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (unpurified)

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 57 kDa
Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Flow cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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