Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11294(B)] to CDK5RAP3 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free
    See all CDK5RAP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11294(B)] to CDK5RAP3 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: IHC-P, IP, WB, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue, Human stomach tissue. Flow Cyt: HEK-293T cells. IP: CDK5RAP3 IP in HEK-293T cells.

  • General notes

    ab242399 is the carrier-free version of ab157203 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab242399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
    Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

    ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab157203 & HEK-293 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

  • Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
    Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
    Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

    Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

  • Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
    Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

    Flow cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green).

    This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

  • Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
    Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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