Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1179Y] to M-CSF - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-M-CSF antibody [EP1179Y] - BSA and Azide free
    See all M-CSF primary antibodies

  • Description

    Rabbit monoclonal [EP1179Y] to M-CSF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue.

  • General notes

    Ab232165 is the carrier-free version of ab52864. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)
    Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

    ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
    Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
    Lane 2 (+): ab52864 & THP-1 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • Flow Cytometry - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)
    Flow Cytometry - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

    Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)
    Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

    Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with ab52864  (1/50 dilution) in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52864).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)
    Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

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