Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-M-CSF antibody [EP1179Y] (ab52864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1179Y] to M-CSF
  • Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-M-CSF antibody [EP1179Y]
    See all M-CSF primary antibodies

  • Description

    Rabbit monoclonal [EP1179Y] to M-CSF

  • Host species

    Rabbit

  • Specificity

    This antibody fails to detect endogenous natural samples in WB.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
    Database link: P09603

  • Positive control

    • WB: Recombinant MCSF IHC-P: Human tonsil tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)

    Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/2000 dilution (Purified) + Human M-CSF (aa 33-190) recombinant protein at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 60 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Flow Cytometry - Anti-M-CSF antibody [EP1179Y] (ab52864)

    Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with unpurified ab52864  (1/50 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/50000 dilution (unpurified) + recombinant MCSF at 0.005 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)
    Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)

    ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
    Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
    Lane 2 (+): ab52864 & THP-1 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-M-CSF antibody [EP1179Y] (ab52864)
    Anti-M-CSF antibody [EP1179Y] (ab52864)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-M-CSF antibody [EP1179Y] (ab52864)

© 2021 Astana Biomed Group LLP. All rights reserved.