Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1179Y] to M-CSF
Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human, Recombinant fragment

Product name

Anti-M-CSF antibody [EP1179Y]
See all M-CSF primary antibodies
Description

Rabbit monoclonal [EP1179Y] to M-CSF
Host species

Rabbit
Specificity

This antibody fails to detect endogenous natural samples in WB.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
Database link: P09603
Positive control
- WB: Recombinant MCSF IHC-P: Human tonsil tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1179Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)

Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)

Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/2000 dilution (Purified) + Human M-CSF (aa 33-190) recombinant protein at 0.015 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 60 kDa
Observed band size: 19 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-M-CSF antibody [EP1179Y] (ab52864)

Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with unpurified ab52864 (1/50 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)

Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/50000 dilution (unpurified) + recombinant MCSF at 0.005 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 60 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)

ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
Lane 2 (+): ab52864 & THP-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Anti-M-CSF antibody [EP1179Y] (ab52864)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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