Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-M-CSF antibody [EPR20948] (ab233387)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20948] to M-CSF
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-M-CSF antibody [EPR20948]
See all M-CSF primary antibodies
Description

Rabbit monoclonal [EPR20948] to M-CSF
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937, P388D1, J774A.1, Jurkat, MDA-MB-231, K562, C6, PC-12 and NIH/3T3 whole cell lysates; Human tonsil, brain and kidney lysates. IHC-P: Human colon and bladder cancer tissues; Mouse and rat colon tissues. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20948
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of human colon (PMID: 15519852; PMID: 11745698).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)

All lanes : Anti-M-CSF antibody [EPR20948] (ab233387) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate
Lanes 3 & 7 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) whole cell lysate
Lane 4 : Human tonsil lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 60 kDa
Observed band size: 43 kDa
why is the actual band size different from the predicted?

Exposure time : Lanes 1-4: 3 minutes; Lanes 5: 6 seconds; Lanes 6-8: 26 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 21502940; PMID: 3264877).
Western blot - Anti-M-CSF antibody [EPR20948] (ab233387)

All lanes : Anti-M-CSF antibody [EPR20948] (ab233387) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : Human brain lysate
Lane 5 : Human kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 60 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?

Exposure time : Lanes 1-3: 3 minutes; Lanes 4-5:10 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 21502940; PMID: 3264877).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Membranous and weak cytoplasmic staining on human bladder cancer (PMID: 25082815; PMID: 25667468) is observed.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of mouse colon (PMID: 15519852; PMID: 11745698).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EPR20948] (ab233387)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling M-CSF with ab233387 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin. Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on stromal cells (arrows) and weak staining on epithelium of rat colon (PMID: 15519852; PMID: 11745698)

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EPR20948] (ab233387)

Immunofluorescent analysis of 100% methanol-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Jurkat cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-M-CSF antibody [EPR20948] (ab233387)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling M-CSF with ab233387 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-M-CSF antibody [EPR20948] (ab233387)

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