Anti-M-CSF antibody (ab99178)
Key features and details
- Rabbit polyclonal to M-CSF
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-M-CSF antibody
See all M-CSF primary antibodies -
Description
Rabbit polyclonal to M-CSF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog, Pig, Macaque monkey, Gorilla
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Spleen; Thymus; Tonsil. It also gave a positive result in FFPE human tonsil tissue sections.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab99178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). Target
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
Post-translational
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 1435 Human
- Entrez Gene: 12977 Mouse
- Omim: 120420 Human
- SwissProt: P09603 Human
- SwissProt: P07141 Mouse
- Unigene: 591402 Human
- Unigene: 795 Mouse
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Alternative names
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody (ab99178)IHC image of MCSF staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99178, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-M-CSF antibody (ab99178)All lanes : Anti-M-CSF antibody (ab99178) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lane 4 : Tonsil (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 170 kDa, 52 kDa (possible cleavage fragment), 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
MCSF contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody (ab99178)This image is courtesy of an anonymous Abreviewab99178 staining MCSF in mouse skin wound tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate-EDTA buffer. Samples were incubated with primary antibody (1/100 in TBS pH 7.6) for 1 hour at 25°C. A Biotin-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/300) was used as the secondary antibody.
Protocols
Datasheets and documents
References (3)
ab99178 has been referenced in 3 publications.
- Mao Z et al. MicroRNA-155 inhibition up-regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate-treated osteoporotic mice. IUBMB Life N/A:N/A (2019). PubMed: 31317664
- Moon HG et al. Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization. Immunity 49:275-287.e5 (2018). PubMed: 30054206
- de Jong RJ et al. Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. Arterioscler Thromb Vasc Biol 37:312-315 (2017). PubMed: 28062503
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody (ab99178)IHC image of MCSF staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99178, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-M-CSF antibody (ab99178)All lanes : Anti-M-CSF antibody (ab99178) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lane 4 : Tonsil (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 170 kDa, 52 kDa (possible cleavage fragment), 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
MCSF contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody (ab99178) This image is courtesy of an anonymous Abreview
ab99178 staining MCSF in mouse skin wound tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate-EDTA buffer. Samples were incubated with primary antibody (1/100 in TBS pH 7.6) for 1 hour at 25°C. A Biotin-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/300) was used as the secondary antibody.
