Recombinant human IL-4 protein (ab155733)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: ELISA, Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.39% MES, 5% Trehalose, 1.17% Sodium chloride, PBS
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
Images
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Functional Studies - Recombinant human IL-4 protein (ab155733)
ab155733 stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.27-0.49 ng/mL
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SDS-PAGE - Recombinant human IL-4 protein (ab155733)Human IL-4 (Tag Free) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. -
ELISA - Recombinant human IL-4 protein (ab155733)Immobilized Human IL-4 (ab155733) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL.
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ELISA - Recombinant human IL-4 protein (ab155733)Ab155733 at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha (ab246188) with a linear range of 2-78 ng/mL.
