Anti-M-CSF antibody (ab9693)
Key features and details
- Rabbit polyclonal to M-CSF
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
Overview
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Product name
Anti-M-CSF antibody
See all M-CSF primary antibodies -
Description
Rabbit polyclonal to M-CSF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Highly pure (>98%) recombinant hM-CSF (human M-CSF)
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Positive control
- Purchase matching WB positive control:Recombinant human M-CSF protein
- WB: Recombinant human M-CSF protein (ab9694).
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab52864
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-M-CSF antibody (ab9693)
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Western blot - Anti-M-CSF antibody (ab9693) This image is courtesy of an Abreview submitted by Valentina MarcelliAll lanes : Anti-M-CSF antibody (ab9693) at 1/500 dilution (for 12 hours at 4°C)
Lane 1 : Human Kidney whole cell lysate
Lane 2 : Human Kidney whole cell lysate transfected with LFABP
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : An IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution
Performed under non-reducing conditions.
Observed band size: 70 kDa why is the actual band size different from the predicted?
Blocking Step: 5% BSA for 1 hour at 25°C
Deatection method: Odyssey for 50 seconds.
Right-hand Image: Weaker exposure to identify bands. -
Sandwich ELISA - Anti-M-CSF antibody (ab9693)Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.
