Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NR2F2 antibody [H7147] (ab41859)

Key features and details

  • Mouse monoclonal [H7147] to NR2F2
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-NR2F2 antibody [H7147]
    See all NR2F2 primary antibodies

  • Description

    Mouse monoclonal [H7147] to NR2F2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment:

    PGQGGPAS TPAQTAAGGQ GGPG

    , corresponding to amino acids 43-64 of Human NR2F2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Convoluted tubule and Rat Glomerular

  • General notes

    This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)

    ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)

    ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-NR2F2 antibody [H7147] (ab41859)
    Western blot - Anti-NR2F2 antibody [H7147] (ab41859)
    All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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