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Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet (ab279048)

Overview

  • Product name

    Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet
    See all Flotillin 1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon10.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

  • Sequence similarities

    Belongs to the band 7/mec-2 family. Flotillin subfamily.

  • Cellular localization

    Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Alternative names

    • FLOT 1
    • FLOT1
    • FLOT1_HUMAN
    • Flotillin-1
    • Flotillin1
    • Integral membrane component of caveolae
    • Reggie 2
    see all

Properties

Images

  • Western blot - Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet (ab279048)
    Western blot - Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet (ab279048)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:FLOT1 knockout HEK293T cell lysate (20 ug)
    Lane 3:A431 cell lysate (20 ug)
    Lane 4:HAP1 cell lysate (20 ug)

    ab78178 was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267276 (knockout cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet (ab279048)
    Western blot - Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet (ab279048)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:FLOT1 knockout HEK293T cell lysate (20 ug)
    Lane 3:HAP1 cell lysate (20 ug)

    ab133497 was shown to specifically react with Flotillin 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267276 (knockout cell lysate ab257109) was used. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab133497 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet (ab279048)
    Sanger Sequencing - Human FLOT1 (Flotillin 1) knockout HEK293T cell pellet (ab279048)
    Homozygous: 8 bp deletion in exon10

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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