Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free
    See all Sarcomeric Alpha Actinin primary antibodies

  • Description

    Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.

  • General notes

    Ab220817 is the carrier-free version of ab68167. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220817 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68167).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68167).

  • Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)
    Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)

    ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10µg)

    Lane 2 (+): ab68167 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68167).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68167).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)
    Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (ab220817)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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