Antibodies, Proteins, Kits and Reagents for Life Science

Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP18708] to SLP-2 - BSA and Azide free
Suitable for: IP, WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-SLP-2 antibody [EP18708] - BSA and Azide free
See all SLP-2 primary antibodies
Description

Rabbit monoclonal [EP18708] to SLP-2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, WB, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human SLP-2 aa 100-200. The exact sequence is proprietary.
Database link: Q9UJZ1
General notes
Ab251098 is the carrier-free version of ab191884. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251098 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EP18708
Isotype

IgG
Research areas

Western blot - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

All lanes : Anti-SLP-2 antibody [EP18708] (ab191884) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

This data was developed using ab191884, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-4: 15 seconds; Lanes 5-7: 5 seconds.
Immunocytochemistry - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

This data was developed using ab191884, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SLP-2 with ab191884 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red). The negative controls are as follows:
-ve control 1: ab191884 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Western blot - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

All lanes : Anti-SLP-2 antibody [EP18708] (ab191884) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 15 seconds

This data was developed using ab191884, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

All lanes : Anti-SLP-2 antibody [EP18708] (ab191884) at 1/2000 dilution

Lane 1 : Human adenocarcinoma of lung tissue lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 15 seconds

This data was developed using ab191884, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

All lanes : Anti-SLP-2 antibody [EP18708] (ab191884) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 3 seconds

This data was developed using ab191884, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

This data was developed using ab191884, the same antibody clone in a different buffer formulation.Flow cytometry analysis of Jurkat cells labelling SLP-2 (red) with purified ab191884 at dilution of 1/900. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Immunoprecipitation - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

This data was developed using ab191884, the same antibody clone in a different buffer formulation.SLP-2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab191884 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab191884 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HeLa whole cell lysate,10ug (Input). Lane 2: ab191884 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191884 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
Immunoprecipitation - Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

This data was developed using ab191884, the same antibody clone in a different buffer formulation.

SLP-2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab191884 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab191884 at 1/1000 dilution (Panel A) or another commercial anti-SLP-2 antibody at 1/1000 dilution (Panel B). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

IP application of ab191884, panel (A), was further validated by performing cross IP with an anti-SLP-2 antibody from another supplier, panel (B).

Lane 1 and 4: Jurkat whole cell lysate 10ug (Input).

Lane 2 and 5: ab191884 IP in Jurkat whole cell lysate.

Lane 3 and 6: Rabbit monoclonal IgG (ab172730) instead of ab191884 in Jurkat whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds (Panels A and B).
Anti-SLP-2 antibody [EP18708] - BSA and Azide free (ab251098)

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