Anti-Calpain 15/SOLH antibody [EPR11078] (ab170926)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11078] to Calpain 15/SOLH
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Calpain 15/SOLH antibody [EPR11078]
See all Calpain 15/SOLH primary antibodies -
Description
Rabbit monoclonal [EPR11078] to Calpain 15/SOLH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human Calpain 15/SOLH aa 1-100. The exact sequence is proprietary.
Database link: O75808 -
Positive control
- Human fetal brain, Human fetal lung, HeLa, 293T and SH-SY5Y lysates; 293T cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Calpain 15
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11078 -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of permeabilized 293T cells labeling Calpain 15/SOLH with ab170926 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
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Anti-Calpain 15/SOLH antibody [EPR11078] (ab170926) at 1/1000 dilution + SH SY5Y cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 117 kDa
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All lanes : Anti-Calpain 15/SOLH antibody [EPR11078] (ab170926) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 117 kDa
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