Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [4A3] to ZBTB20 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-ZBTB20 antibody [4A3] - BSA and Azide free
    See all ZBTB20 primary antibodies

  • Description

    Rat monoclonal [4A3] to ZBTB20 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Fusion protein (His-tag) corresponding to Mouse ZBTB20.
    Database link: Q8K0L9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Mouse E14.5 cerebrum tissue. Mouse and rat hippocampus. Mouse pancreas tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252244 is the carrier-free version of ab243143. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 cerebrum tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse E14.5 cerebrum(PMID: 20308569). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243143).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse pancreas (PMID: 22374165). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243143).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse hippocampus (PMID: 11744704). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243143).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat hippocampus (PMID: 11744704). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243143).

  • Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)
    Anti-ZBTB20 antibody [4A3] - BSA and Azide free (ab252244)

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