Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZBTB20 antibody [4A3] (ab243143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [4A3] to ZBTB20
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-ZBTB20 antibody [4A3]
    See all ZBTB20 primary antibodies

  • Description

    Rat monoclonal [4A3] to ZBTB20

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Fusion protein (His-tag) corresponding to Mouse ZBTB20.
    Database link: Q8K0L9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Mouse E14.5 cerebrum tissue. Mouse and rat hippocampus. Mouse pancreas tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Ion Exchange Chromatography

  • Clonality

    Monoclonal

  • Clone number

    4A3

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 cerebrum tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse E14.5 cerebrum(PMID: 20308569). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse pancreas (PMID: 22374165). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse hippocampus (PMID: 11744704). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat hippocampus (PMID: 11744704). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-ZBTB20 antibody [4A3] (ab243143)
    Anti-ZBTB20 antibody [4A3] (ab243143)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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