Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸ 10 µg 110 563 ₸

Order now and get it on Tuesday March 16, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [4A3] to ZBTB20
Suitable for: IHC-P
Reacts with: Mouse, Rat

Product name

Anti-ZBTB20 antibody [4A3]
See all ZBTB20 primary antibodies
Description

Rat monoclonal [4A3] to ZBTB20
Host species

Rat
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

See all applications and species data
Immunogen

Fusion protein (His-tag) corresponding to Mouse ZBTB20.
Database link: Q8K0L9

Run BLAST with Run BLAST with
Positive control
- IHC-P: Mouse E14.5 cerebrum tissue. Mouse and rat hippocampus. Mouse pancreas tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Ion Exchange Chromatography
Clonality

Monoclonal
Clone number

4A3
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

Immunohistochemical analysis of paraffin-embedded mouse E14.5 cerebrum tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse E14.5 cerebrum(PMID: 20308569). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse pancreas (PMID: 22374165). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse hippocampus (PMID: 11744704). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB20 antibody [4A3] (ab243143)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat hippocampus (PMID: 11744704). Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-ZBTB20 antibody [4A3] (ab243143)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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