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Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)

Key features and details

  • Mouse monoclonal [ACTN2/3292] to Sarcomeric Alpha Actinin - BSA and Azide free
  • Suitable for: Protein Array, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free
    See all Sarcomeric Alpha Actinin primary antibodies

  • Description

    Mouse monoclonal [ACTN2/3292] to Sarcomeric Alpha Actinin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Sarcomeric Alpha Actinin aa 557-692. The exact sequence is proprietary.
    Database link: P35609

  • Positive control

    • IHC-P: Human cardiac muscle and skeletal muscle tissue. WB: Human skeletal muscle tissue lysate.

  • General notes

    ab257328 is a PBS only version of ab257317.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)

    Formalin-fixed, paraffin-embedded human cardiac muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis. 

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    This data was produced with ab257317, the same antibody in a different formulation with BSA and Azide.

  • Western blot - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)
    Western blot - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)
    Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] (ab257317) at 2 µg/ml + Human skeletal muscle tissue lysate

    Predicted band size: 103 kDa



    This data was produced with ab257317, the same antibody in a different formulation with BSA and Azide.

  • Protein Array - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)
    Protein Array - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)

    Analysis of >19,00 full-length human proteins using ab257317.

    This data was produced with ab257317, the same antibody in a different formulation with BSA and Azide.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)

    Formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis. 

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    This data was produced with ab257317, the same antibody in a different formulation with BSA and Azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [ACTN2/3292] - BSA and Azide free (ab257328)

    Formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling Sarcomeric Alpha Actinin using ab257317 at 2 μg/ml in immunohistochemical analysis. 

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    This data was produced with ab257317, the same antibody in a different formulation with BSA and Azide.

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