Antibodies, Proteins, Kits and Reagents for Life Science

365 193 ₸ Product size

100 µl 365 193 ₸ 10 µl 150 768 ₸

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Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y]
See all Sarcomeric Alpha Actinin primary antibodies
Description

Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2529Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Human fetal heart tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Blocking and dilution buffer: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.

Lane 1 (input): HepG2 whole cell lysate (10µg)

Lane 2 (+): ab68167 + HepG2 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.

All lanes :

Lane 1 : HepG2 whole cell lysate at 10 µg
Lane 2 : ab68167 + HepG2 whole cell lysate at 10 µg
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate

Observed band size: 103 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (unpurified)

Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat heart lysate
Lane 3 : Human heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Mouse heart tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Blocking and dilution buffer: 5% NFDM /TBST.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/10000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Blocking and dilution buffer: 5% NFDM /TBST.
Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

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