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Human FSCN1 (Fascin) knockout HCT116 cell pellet (ab278993)

Overview

  • Product name

    Human FSCN1 (Fascin) knockout HCT116 cell pellet
    See all Fascin kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HCT116

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the fascin family.

  • Domain

    Composed of four beta-trefoil domains.

  • Post-translational
    modifications

    Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium.
  • Information by UniProt

  • Alternative names

    • 55 kDa actin bundling protein
    • 55 kDa actin-bundling protein
    • Actin bundling protein
    • actin bundling protein, 55-KD
    • FAN 1
    • FAN1
    • Fascin
    • Fascin 1
    • Fascin actin bundling protein 1
    • Fascin homolog 1
    • Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus)
    • Fascin, sea urchin, homolog of, 1
    • Fascin1
    • FLJ38511
    • FSCN 1
    • FSCN1
    • FSCN1_HUMAN
    • HSN
    • p55
    • Singed (Drosophila) like (sea urchin fascin homolog like)
    • Singed drosophila homolog like
    • Singed like (fascin homolog sea urchin)
    • Singed like (fascin homolog sea urchin) (Drosophila)
    • Singed like protein
    • Singed, drosophila, homolog of
    • Singed-like protein
    • SNL
    • Strongylocentrotus purpuratus
    see all

Properties

Images

  • Western blot - Human FSCN1 (Fascin) knockout HCT116 cell pellet (ab278993)
    Western blot - Human FSCN1 (Fascin) knockout HCT116 cell pellet (ab278993)

    Lane 1:Wild-type HCT116 cell lysate (20 ug)
    Lane 2:FSCN1 knockout HCT116 cell lysate (20 ug)
    Lane 3:SH-SY5Y cell lysate (20 ug)
    Lane 4:K-562 cell lysate (20 ug)

    ab126772 was shown to specifically react with Fascin in wild-type HCT116 cells. Loss of signal was observed when knockout cell line ab266895 (knockout cell lysate ab257444) was used. Wild-type and Fascin knockout samples were subjected to SDS-PAGE. ab126772 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell pellet (ab278993)
    Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell pellet (ab278993)

    Allele-1: 1 bp deletion in exon1

     

  • Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell pellet (ab278993)
    Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell pellet (ab278993)

    Allele-2: Insertion of the selection cassette in exon1

     

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