Anti-Sarcomeric Alpha Actinin antibody (ab90776)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit polyclonal to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow
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Immunogen
Synthetic peptide corresponding to Human Sarcomeric Alpha Actinin aa 850 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab100828) -
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Skeletal Muscle; Heart.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab90776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 396263 Chicken
- Entrez Gene: 536607 Cow
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Omim: 102573 Human
- SwissProt: Q3ZC55 Cow
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
see all -
Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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Western blot - Anti-Sarcomeric Alpha Actinin antibody (ab90776)All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab90776) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 27 kDa, 30 kDa, 45 kDa, 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (ab90776)IHC image of Sarcomeric Alpha Actinin staining in human Heart FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90776, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Protocols
References (5)
ab90776 has been referenced in 5 publications.
- Wheelwright M et al. Advancing physiological maturation in human induced pluripotent stem cell-derived cardiac muscle by gene editing an inducible adult troponin isoform switch. Stem Cells N/A:N/A (2020). PubMed: 32497296
- Huang Y et al. Age-Dependent Oxidative DNA Damage Does Not Correlate with Reduced Proliferation of Cardiomyocytes in Humans. PLoS One 12:e0170351 (2017). PubMed: 28099512
- Ma ZG et al. Piperine Attenuates Pathological Cardiac Fibrosis Via PPAR-?/AKT Pathways. EBioMedicine 18:179-187 (2017). PubMed: 28330809
- Gavin KM et al. Hematopoietic-to-mesenchymal transition of adipose tissue macrophages is regulated by integrin ß1 and fabricated fibrin matrices. Adipocyte 6:234-249 (2017). PubMed: 28441086
- Liang P et al. Patient-Specific and Genome-Edited Induced Pluripotent Stem Cell-Derived Cardiomyocytes Elucidate Single-Cell Phenotype of Brugada Syndrome. J Am Coll Cardiol 68:2086-2096 (2016). ICC/IF ; Human . PubMed: 27810048
Images
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Western blot - Anti-Sarcomeric Alpha Actinin antibody (ab90776)All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab90776) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 27 kDa, 30 kDa, 45 kDa, 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (ab90776)IHC image of Sarcomeric Alpha Actinin staining in human Heart FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90776, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
