Anti-Sarcomeric Alpha Actinin antibody (ab72592)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit polyclonal to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the N-terminal of human Sarcomeric Alpha Actinin.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab72592) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HUVEC cells
Lane 4 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 103 kDa
Observed band size: 103 kDa
Additional bands at: 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (ab72592)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab72592, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Immunofluorescent analysis of HeLa cells using ab72592, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising peptide.