Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)
![Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)](https://www.abcam.com/ps/products/249/ab249146/Images/ab249146-450549-anti-atg4b-antibody-epr64362-bsa-and-azide-free-western-blot-wildetype-HeLa-atg4b-knockout-HeLa.jpg "Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6436(2)] to ATG4B - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free
See all ATG4B primary antibodies -
Description
Rabbit monoclonal [EPR6436(2)] to ATG4B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Human ATG4B protein (ab123195) can be used as a positive control in WB. Ramos, 293T, Jurkat and HeLa cell lysates; Jurkat cells.
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General notes
Ab249146 is the carrier-free version of ab154843. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249146 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6436(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)All lanes : Anti-ATG4B antibody [EPR6436(2)] (ab154843) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATG4B knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab154843).
Lanes 1 - 2: Merged signal (red and green). Green - ab154843 observed at 47 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab154843 was shown to react with ATG4B in wild-type HEK293T cells in Western blot with loss of signal observed in ATG4B knockout cell line ab265814 (ATG4B knockout cell lysate ab257364). Wild-type and ATG4B knockout HEK293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154843 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)](https://www.abcam.com/ps/products/249/ab249146/Images/ab249146-406430-anti-atg4b-antibody-epr6436-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)This data was developed using ab154843, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: ATG4B knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab154843 observed at 47 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab154843 was shown to specifically react with ATG4B when ATG4B knockout samples were used. Wild-type and ATG4B knockout samples were subjected to SDS-PAGE. ab154843 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)](https://www.abcam.com/ps/products/249/ab249146/Images/ab249146-402392-anti-atg4b-antibody-epr6436-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)All lanes : Anti-ATG4B antibody [EPR6436(2)] (ab154843) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDaThis data was developed using ab154843, the same antibody clone in a different buffer formulation.
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![Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)](https://www.abcam.com/ps/products/249/ab249146/Images/ab249146-3-benefits-of-recombinant-antibodies.png)
Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free (ab249146)
