Recombinant human FES protein (ab196084)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human FES protein
See all FES proteins and peptides -
Biological activity
Specific activity of 235 pmol/min/µg.
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Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHM SLQDSGGQSRAISPDSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSK LSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKSQYRALARDSAQ AKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHH QLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAA AARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNE LTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRE RVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPV LLLQDDRHSTSSSEQEREGGR TPTLEILKSHISGIFRPKFSLPPPLQL IPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQEYVLSVLW DGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHR AVPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLP PDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTF LRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVL KISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFG ILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCW AYEPGQRPSFSTIYQELQSIRKRHR -
Predicted molecular weight
95 kDa including tags -
Amino acids
2 to 822 -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
Genbank #: NM_002005
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.64% Tris HCl, 0.64% Sodium chloride, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.017% Potassium chloride, 20% Glycerol (glycerin, glycerine)
Buffer also contains FLAG peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human FES protein (ab196084)
Analysis of ATP transfer with various amounts of ab196084. Assay was performed in a Kinase buffer (40 mM Tris pH 7.4, 10 mM MgCl2, 0.1 mg/mL BSA) containing 1 mM DTT using Poly-(Glu4:Tyr)substrate (0.2 mg/mL) and 20 µM ATP for 30 minutes at 30°C.
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SDS-PAGE - Recombinant human FES protein (ab196084)10% SDS-PAGE analysis of ab196084 stained with Coomassie Blue.
