Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)
![Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)](https://www.abcam.com/ps/products/32/ab32569/Images/ab32569-269788-anti-pi3-kinase-p110-beta-antibody-y384-western-blot.jpg "Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y384] to PI3 Kinase p110 beta
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PI3 Kinase p110 beta antibody [Y384]
See all PI3 Kinase p110 beta primary antibodies -
Description
Rabbit monoclonal [Y384] to PI3 Kinase p110 beta -
Host species
Rabbit -
Specificity
ab32569 recognises PI3-Kinases (PI3-Ks). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PI3 Kinase p110 beta. The exact sequence is proprietary.
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Positive control
- Jurkat whole cell lysate (ab7899).
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
Y384 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: PI3 Kinase p110 beta knockout HAP1 cell lysate (40 µg)
Lane 3: Jurkat cell lysate (40 µg)
Lane 4: K562 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32569 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.ab32569 was shown to recognize PI3 Kinase p110 beta when PI3 Kinase p110 beta knockout samples were used, along with additional cross-reactive bands. Wild-type and PI3 Kinase p110 beta knockout samples were subjected to SDS-PAGE. Ab32569 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)](https://www.abcam.com/ps/products/32/ab32569/Images/ab32569-45272-anti-pi3-kinase-p110-beta-antibody-y384-western-blot.jpg)
Western blot - Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 123 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
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![Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)](https://www.abcam.com/ps/products/32/ab32569/Images/ab32569-3-benefits-of-recombinant-antibodies.png)
Anti-PI3 Kinase p110 beta antibody [Y384] (ab32569)
