Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5515(2)] to PI3 Kinase p110 beta
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]
See all PI3 Kinase p110 beta primary antibodies
Description

Rabbit monoclonal [EPR5515(2)] to PI3 Kinase p110 beta
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human PI3 Kinase p110 beta aa 400-500. The exact sequence is proprietary.
Positive control
- MCF-7, Jurkat, K562 and 293T cell lysates; Human brain tissue; 293 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5515(2)
Isotype

IgG
Research areas

Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

Lane 1 : Rat Brain Lysate
Lane 2 : Mouse Brain Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 123 kDa
Flow Cytometry - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Overlay histogram showing SH-SY5Y cells stained with ab131260 (red line) at 1/20 dilution. The cells were fixed with 2% Paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.
Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

ab151549 staining PI3 Kinase p110 beta in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/75). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/5000 dilution + K562 Cell Lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 123 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

Lane 1 : 293 Cell Lysate
Lane 2 : Jurkat Cell Lysate
Lane 3 : MCF-7 Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 123 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Overlay histogram showing HeLa cells stained with unpurified ab151549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151549, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution (unpurified)

Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 123 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using unpurified ab151549 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151549 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using unpurified ab151549 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151549 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab151549 showing +ve staining.
Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549)

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