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Recombinant Human Podoplanin / gp36 protein (ab152053)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    pH: 7.20
    Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Dissolve the lyophilized protein in 1X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days. For long term storage aliquot and store at

    General Info

    • Alternative names

      • Aggrus
      • Glycoprotein 36 KD
      • Glycoprotein 36
      • gp 36
      • GP 38
      • GP 40
      • gp36
      • GP38
      • GP40
      • HT1A 1
      • HT1A1
      • hT1alpha 1
      • hT1alpha 2
      • hT1alpha1
      • hT1alpha2
      • Lung type I cell membrane associated glycoprotein
      • Lung type I cell membrane associated glycoprotein isoform a
      • Lung type I cell membrane associated glycoprotein T1A 2
      • OTS 8
      • OTS8
      • OTTHUMP00000009640
      • OTTHUMP00000044504
      • PA2.26
      • PA2.26 antigen
      • Pdpn
      • PDPN_HUMAN
      • Podoplanin
      • PSEC0003
      • PSEC0025
      • T1 alpha
      • T1 ALPHA GENE
      • T1-alpha
      • T1A
      • T1A 2
      • TI1A
      • TIA 2
      • TIA2
      see all

    • Function

      May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.

    • Tissue specificity

      Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.

    • Sequence similarities

      Belongs to the podoplanin family.

    • Post-translational
      modifications

      Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.

    • Cellular localization

      Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

References (0)

Publishing research using ab152053? Please let us know so that we can cite the reference in this datasheet.

ab152053 has not yet been referenced specifically in any publications.

Images

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