Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hemoglobin antibody [7E1F] (ab77125)

Key features and details

  • Mouse monoclonal [7E1F] to Hemoglobin
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Hemoglobin antibody [7E1F]
    See all Hemoglobin primary antibodies

  • Description

    Mouse monoclonal [7E1F] to Hemoglobin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Human plasma

  • Positive control

    • Human plasma.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 20th January 2020. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Hemoglobin antibody [7E1F] (ab77125)
    Western blot - Anti-Hemoglobin antibody [7E1F] (ab77125)
    All lanes : Anti-Hemoglobin antibody [7E1F] (ab77125) at 1/1000 dilution

    Lane 1 : Hemoglobin isolated from Human
    plasma
    Lane 2 : Human plasma at 1 µl

    Predicted band size: 15 kDa



    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Hemoglobin antibody [7E1F] (ab77125)
    Flow Cytometry - Anti-Hemoglobin antibody [7E1F] (ab77125)

    Overlay histogram showing K562 cells stained with ab77125 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77125, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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