Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 03, 2021

Anti-Hemoglobin antibody [7E1F] (ab77125)

Key features and details

Mouse monoclonal [7E1F] to Hemoglobin
Suitable for: WB, Flow Cyt
Reacts with: Human
Isotype: IgG1

Product name

Anti-Hemoglobin antibody [7E1F]
See all Hemoglobin primary antibodies
Description

Mouse monoclonal [7E1F] to Hemoglobin
Host species

Mouse
Tested applications

Suitable for: WB, Flow Cytmore details
Species reactivity

Reacts with: Human
Immunogen

Human plasma
Positive control
- Human plasma.
General notes

This product was changed from ascites to tissue culture supernatant on 20th January 2020. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.03% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, HEPES
Concentration information loading...
Purity

IgG fraction
Clonality

Monoclonal
Clone number

7E1F
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-Hemoglobin antibody [7E1F] (ab77125)

All lanes : Anti-Hemoglobin antibody [7E1F] (ab77125) at 1/1000 dilution

Lane 1 : Hemoglobin isolated from Human
plasma
Lane 2 : Human plasma at 1 µl

Predicted band size: 15 kDa

This image was generated using the ascites version of the product.
Flow Cytometry - Anti-Hemoglobin antibody [7E1F] (ab77125)

Overlay histogram showing K562 cells stained with ab77125 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77125, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

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