Mouse CXCL16 Matched Antibody Pair Kit (ab211757)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.6 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse CXCL16 Matched Antibody Pair Kit
See all CXCL16 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.57 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CXCL16 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CXCL16.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab197742. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse CXCL16 Capture Antibody 2 x 50µg 1 x 50µg Mouse CXCL16 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse CXCL16 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. -
Tissue specificity
Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble form. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 16
- Chemokine (C X C motif) ligand 16
- Chemokine, CXC motif, ligand 16
see all -
Database links
- Entrez Gene: 66102 Mouse
- SwissProt: Q8BSU2 Mouse
- Unigene: 425692 Mouse
Images
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Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse CXCL16 Matched Antibody Pair Kit (ab211757)To learn more about the advantages of recombinant antibodies see here.
