Mouse CXCL16 ELISA Kit (ab197744)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.6 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CXCL16 ELISA Kit
See all CXCL16 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% RAW 264.7 5 6.3% Inter-assay Sample n Mean SD CV% RAW 264.7 3 3.2% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.6 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99.9 84.8% - 112.5% Cell culture media 96.7 86% - 108.3% Cit plasma 90.5 84.2% - 95.8% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse CXCL16 ELISA Kit (ab197744) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL16 with 7.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CXCL16 is a chemokine. It induces a strong chemotactic response. It induces calcium mobilization. CXCL16 binds to CXCR6/Bonzo. CXCL16 also acts as a scavenger receptor on macrophages, which specifically binds to oxidized low density lipoprotein, suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 is a plasma membrane protein.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CXCL16 Capture Antibody 1 x 600µl 10X Mouse CXCL16 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Mouse CXCL16 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. It induces a strong chemotactic response and calcium mobilization. It binds to CXCR6/Bonzo. -
Cellular localization
Cell Membrane and Secreted -
Alternative names
- 0910001K24Rik
- AV290116
- BB024863
see all -
Database links
- Entrez Gene: 66102 Mouse
- SwissProt: Q8BSU2 Mouse
- Unigene: 425692 Mouse
Images
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Other - Mouse CXCL16 ELISA Kit (ab197744)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of CXCL16 standard curve prepared in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of CXCL16 standard curve prepared in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CXCL16 in mouse serum and plasma (citrate) samples.The concentrations of CXCL16 were measured in duplicates, interpolated from the CXCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10% and plasma (citrate) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native CXCL16 in mouse LPS-treated RAW264.7 supernatant samples.RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of CXCL16 were measured in duplicates, interpolated from the CXCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 supernatant 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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CXCL16 concentrations in unstimulated and LPS-stimulated RAW 264.7 cell culture supernatants.RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. Two dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of CXCL16 adjusted for sample dilution are graphed in pg of CXCL16 per mL of supernatant (mean +/- SD, n = 2).
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Sandwich ELISA - Mouse CXCL16 ELISA Kit (ab197744)To learn more about the advantages of recombinant antibodies see here.
