Caspase Assay Kit (Flow Cytometry) (ab273272)
Key features and details
- Assay type: Cell-based
- Detection method: Flow cytometry-fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Caspase Assay Kit (Flow Cytometry)
See all Caspase kits -
Detection method
Flow cytometry-fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Assay duration
Multiple steps standard assay -
Product overview
Caspase Assay Kit (Flow cytometry) (ab273272) provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm. As the D2R is more cell-permeable than other fluorometric caspase substrates, activation of caspases can easily be measured in intact cells by flow cytometry.
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Tested applications
Suitable for: Flow Cytmore details -
Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 25 tests D2R Incubation Buffer 1 x 25µl D2R Reagent 1 x 25µl DTT 1 x 125µl -
Research areas
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Relevance
Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. -
Cellular localization
Cytoplasmic
