Anti-SOX5 antibody (ab94396)
Key features and details
- Rabbit polyclonal to SOX5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SOX5 antibody -
Description
Rabbit polyclonal to SOX5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseChickenCowPigZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).Target
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Function
Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6660 Human
- Entrez Gene: 20678 Mouse
- Omim: 604975 Human
- SwissProt: P35711 Human
- SwissProt: P35710 Mouse
- Unigene: 657542 Human
- Unigene: 1752 Mouse
- Unigene: 482428 Mouse
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Alternative names
- L SOX5 antibody
- MGC35153 antibody
- Sex determining region Y box 5 antibody
see all
Images
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Western blot - Anti-SOX5 antibody (ab94396)Anti-SOX5 antibody (ab94396) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 24 kDa, 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Protocols
Datasheets and documents
References (14)
ab94396 has been referenced in 14 publications.
- Takimoto A et al. Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation. Sci Rep 9:4605 (2019). PubMed: 30872687
- Sun C et al. SOX5 promotes breast cancer proliferation and invasion by transactivation of EZH2. Oncol Lett 17:2754-2762 (2019). PubMed: 30854049
- DiNuoscio G & Atit RP Wnt/ß-catenin signaling in the mouse embryonic cranial mesenchyme is required to sustain the emerging differentiated meningeal layers. Genesis 57:e23279 (2019). PubMed: 30615824
- Yano F et al. Runx1 contributes to articular cartilage maintenance by enhancement of cartilage matrix production and suppression of hypertrophic differentiation. Sci Rep 9:7666 (2019). PubMed: 31113964
- Carr MJ et al. Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration. Cell Stem Cell 24:240-256.e9 (2019). PubMed: 30503141
Images
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Western blot - Anti-SOX5 antibody (ab94396)Anti-SOX5 antibody (ab94396) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 24 kDa, 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
