Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free (ab232450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YE351] to PP2A alpha + beta - BSA and Azide free
  • Suitable for: Dot blot, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free
    See all PP2A alpha + beta primary antibodies

  • Description

    Rabbit monoclonal [YE351] to PP2A alpha + beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab232450 reacts with an epitope located in the N terminal region of PP2A alpha.

  • Positive control

    • IHC-P: Human tonsil tissue. Dot Blot: PP2A-alpha peptide and PP2A-beta peptide.

  • General notes

    Ab232450 is the carrier-free version of ab32065. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232450 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free (ab232450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free (ab232450)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    Cytoplasm and weak nucleus staining is visible in lymphocytes of human tonsil.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32065).

  • Dot Blot - Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free (ab232450)
    Dot Blot - Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free (ab232450)

    This data was developed using the same antibody clone in a different buffer formulation (ab32065).

    Dot blot analysis of PP2A-alpha peptide (Lane 1) and PP2A-beta peptide (Lane 2) labelling PP2A alpha+beta with ab32065 at a dilution of 1/1000. A HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at a dilution of 1/1000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free (ab232450)
    Anti-PP2A alpha + beta antibody [YE351] - BSA and Azide free (ab232450)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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