Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Collagen V antibody [EPR23762-54] (ab275881)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23762-54] to Collagen V
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Collagen V antibody [EPR23762-54]
    See all Collagen V primary antibodies

  • Description

    Rabbit monoclonal [EPR23762-54] to Collagen V

  • Host species

    Rabbit

  • Specificity

    The immunogen of this antibody is based on Collagen V-COL5A2, low homology with Collagen V-COL5A1 based on immunogen design.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat and mouse articular cartilage tissue lysate; Rat and Mouse articular cartilage organ culture supernatant.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23762-54

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Collagen V antibody [EPR23762-54] (ab275881)
    Western blot - Anti-Collagen V antibody [EPR23762-54] (ab275881)
    All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution

    Lane 1 : Mouse articular cartilage organ culture supernatant, 10 ul
    Lane 2 : Mouse articular cartilage organ culture supernatant treated with PNGase F, 10 ul
    Lane 3 : Mouse articular cartilage tissue lysate, 20ug
    Lane 4 : Mouse articular cartilage tissue lysate treated with PNGase F, 20ug

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 184 kDa
    Observed band size: 32,36 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 20625483).

    Glycosylation leads to the shift of the bands.

    Exposure time: 6 seconds.

     

  • Western blot - Anti-Collagen V antibody [EPR23762-54] (ab275881)
    Western blot - Anti-Collagen V antibody [EPR23762-54] (ab275881)
    All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution

    Lane 1 : Rat placenta organ culture supernatant 30 ul
    Lane 2 : Rat articular cartilage tissue lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 184 kDa
    Observed band size: 145,32 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 23262228, 20625483).

    Exposure time: Lane 1: 92 seconds Lane 2: 1 seconds.

     

  • Western blot - Anti-Collagen V antibody [EPR23762-54] (ab275881)
    Western blot - Anti-Collagen V antibody [EPR23762-54] (ab275881)
    All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution

    Lane 1 : Mouse articular cartilage tissue lysate
    Lane 2 : Mouse PBMCs (peripheral blood mononuclear cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 184 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 20625483).

    Low expression: Mouse PBMCs (database:HPA).

    Exposure time: Lane 1: 5 seconds Lane 2: 3 minutes.

     

  • Anti-Collagen V antibody [EPR23762-54] (ab275881)
    Anti-Collagen V antibody [EPR23762-54] (ab275881)

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