Anti-FANCI (phospho S559) antibody (ab228518)
Key features and details
- Rabbit polyclonal to FANCI (phospho S559)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FANCI (phospho S559) antibody
See all FANCI primary antibodies -
Description
Rabbit polyclonal to FANCI (phospho S559) -
Host species
Rabbit -
Specificity
Specific for endogenous levels of the ~150 kDa FANCI protein phosphorylated at Ser559. Immunolabeling is increased when HeLa cells are treated with 256nm UV light for 20 minutes and completely eliminated by treatment with lambda phosphatase. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human FANCI (phospho S559).
Database link: Q9NVI1 -
Positive control
- WB: HeLa cell lysate treated with UV (~254 nm) for 20 minutes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.01% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab228518 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FANCI (phospho S559) antibody (ab228518)All lanes : Anti-FANCI (phospho S559) antibody (ab228518) at 1/250 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 20 minutes
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 20 minutes followed by treatment with lambda phosphatase (1200 units for 30 min)
Predicted band size: 149 kDa
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Western blot - Anti-FANCI (phospho S559) antibody (ab228518)All lanes : Anti-FANCI (phospho S559) antibody (ab228518) at 1/250 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 0 minutes
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 20 minutes
Predicted band size: 149 kDa
