Anti-SIRP alpha antibody (ab232765)
Key features and details
- Rabbit polyclonal to SIRP alpha
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SIRP alpha antibody
See all SIRP alpha primary antibodies -
Description
Rabbit polyclonal to SIRP alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human SIRP alpha aa 100-350. Expressed in E.coli. N-terminal tags.
Database link: P78324-1 -
Positive control
- WB: Recombinant human SIRP alpha protein; HEK-293T cell lysate; Pig brain lysate. IHC-P: Human cerebrum, liver, breast cancer and stomach tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (ab232765)
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-SIRP alpha antibody (ab232765)All lanes : Anti-SIRP alpha antibody (ab232765) at 2 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 55 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (ab232765)Formalin-fixed, paraffin-embedded human liver tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (ab232765)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRP alpha antibody (ab232765)Formalin-fixed, paraffin-embedded human stomach tissue stained for SIRP alpha using ab232765 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-SIRP alpha antibody (ab232765)Anti-SIRP alpha antibody (ab232765) at 2 µg + Recombinant human SIRP alpha protein
Predicted band size: 55 kDa
