Anti-EGFR (phospho S967) antibody (ab76193)
Key features and details
- Rabbit polyclonal to EGFR (phospho S967)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EGFR (phospho S967) antibody
See all EGFR primary antibodies -
Description
Rabbit polyclonal to EGFR (phospho S967) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding serine 967 of human EGFR conjugated to KLH
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Positive control
- WB: A431 cells treated with Calyculin A (100 nM) for 30 minutes. IHC-P: A431 cells untreated or treated with Calyculin A (50 nM) for 15 minutes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76193 was cross adsorbed to unphosphorylated EGFR (Ser-967), coupled to agarose then affinity purified using phospho EGFR (Ser-967) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho S967) antibody (ab76193)All lanes : Anti-EGFR (phospho S967) antibody (ab76193) at 1/1000 dilution
Lane 1 : human A431 cells treated with
Calyculin A (100 nM) for 30 min
Lane 2 : human A431 cells treated with
Calyculin A (100 nM) for 30 min and treated with lambda phosphatase
Predicted band size: 134 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S967) antibody (ab76193)ab76193, at a 1/200 dilution, staining EGFR in A431 cells by Immunofluorescence.
Left image: untreated cells
Middle image: cells treated with Calyculin A (50 nM) for 15 minutes
Right image: cells treated with Calyculin A (50 nM) for 15 minutes, with the blocking peptide phospho EGFR (serine 967).
