Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL12] to Met (c-Met) - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [CAL12] to Met (c-Met) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human non-small cell lung carcinoma and lung carcinoma tissue. ICC/IF: NCI-H1648 cells.

  • General notes

    Ab251598 is the carrier-free version of ab237711. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251598 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Met (c-Met) with ab237711 at 1:1000 dilution (0.45 μg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human lung carcinoma.

    The section was incubated with ab237711 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237711).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)

    Formalin-fixed, paraffin-embedded human non-small cell lung carcinoma tissue stained for Met (c-Met) using ab237711 at 1 μg/ml in immunohistochemical analysis.

    Incubate with primary antibody for 75 minutes at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237711).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)

    Formalin-fixed, paraffin-embedded human non-small cell lung carcinoma tissue stained for Met (c-Met) using ab237711 at 1 μg/ml in immunohistochemical analysis.

    Incubate with primary antibody for 75 minutes at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237711).

  • Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)
    Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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