Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2367Y] to Met (c-Met) (phospho Y1349)
  • Suitable for: Dot blot, WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y]
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [EP2367Y] to Met (c-Met) (phospho Y1349)

  • Host species

    Rabbit

  • Specificity

    This antibody detects Met (c-Met) phosphorylated at tyrosine 1349.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Met (c-Met). The exact sequence is proprietary.
    (Peptide available as ab175821)

  • Positive control

    • WB: HeLa and A431 cell lysate IHC: Human breast carcinoma tissue IP: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68141 at a dilution of 1/50.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    All lanes : Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141) at 1/5000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h. Whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h and then treated with hepatocyte growth factor at 40ng/ml for 5 min. Whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h and then treated with hepatocyte growth factor at 40ng/ml for 5 min. Whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 156 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight of Met (c-Met) (phospho Y1349) and Met (c-Met) is different. Ab51067 could only recognize the pro-Met which is 190kDa, but ab68141 recognizes theβ-subunits which is 145kDa.

  • Immunoprecipitation - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    Immunoprecipitation - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)

    ab68141 at 1/150 dilution immunoprecipitating Met (c-Met) (phospho Y1349) in HeLa (human cervix adenocarcinoma) whole cell lysate observed at 150 kDa (lanes 1 and 2).

    Lane 1 (input): HeLa cells starved for 24 hours, then treated with 40 ng/mL HGF for 5 minutes whole cell lysate, 10μg
    Lane 2 (+): ab68141 + HeLa cells starved for 24 hours, then treated with 40 ng/mL HGF for 5 minutes whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68141 in HeLa cells starved for 24 hours, then treated with 40 ng/mL HGF for 5 minutes whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    Western blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    All lanes : Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141) at 1/10000 dilution

    Lane 1 : A431 cell lysate, untreated
    Lane 2 : A431 cell lysate, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 156 kDa
    Observed band size: ~150 kDa why is the actual band size different from the predicted?

  • Dot Blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    Dot Blot - Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)

    Dot blot analysis of Met (c-Met) (phospho Y1349) phospho peptide (Lane 1), Met (c-Met) Non-phospho peptide (Lane 2), labelling Met (c-Met) (phospho Y1349) with ab68141 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)
    Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] (ab68141)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.