Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP44] to Met (c-Met) - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Met (c-Met) antibody [SP44] - BSA and Azide free
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [SP44] to Met (c-Met) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon carcinoma tissue. Flow Cyt: HAP1, HT-29 and HepG2 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab243930 is the carrier-free version of ab227637. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243930 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Met (c-Met) with ab227637 at 1/50 dilution (4.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227637)
  • Flow Cytometry - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
    Flow Cytometry - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)

    This data was developed using the same antibody clone in a different buffer formulation (ab227637).

    Flow cytometry overlay histogram showing wild-type HAP1 (green line) and MET knockout HAP1 cells stained with ab227637 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab227637) (1x106 in 100 µl at 0.04 µg/ml) for 30 min at 22°C.

    The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 22°C.

    Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line MET knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Met (c-Met) using ab227637 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227637).

  • Flow Cytometry - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
    Flow Cytometry - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Met (c-Met) with purified ab227637 at 1/20 dilution (11.3 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227637).

  • Flow Cytometry - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
    Flow Cytometry - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)

    Flow cytometric analysis of HT-29 (human colorectal adenocarcinoma cell line) cell line labeling Met (c-Met) with ab227637 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227637).

  • Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
    Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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