Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free (ab254474)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19554-110] to Met (c-Met) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [EPR19554-110] to Met (c-Met) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: B16-F10 whole cell lysate

  • General notes

    Ab254474 is the carrier-free version of ab254252. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254474 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free (ab254474)
    Western blot - Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free (ab254474)
    Anti-Met (c-Met) antibody [EPR19554-110] (ab254252) at 1/1000 dilution + B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 155 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID 8710887).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254252).

  • Western blot - Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free (ab254474)
    Western blot - Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free (ab254474)
    All lanes : Anti-Met (c-Met) antibody [EPR19554-110] (ab254252) at 1/1000 dilution

    Lane 1 : Untreated B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate
    Lane 2 : B16-F10 whole cell lysate treated with PNGase F

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 155 kDa
    Observed band size: 175 kDa why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    c-Met is an N-linked glycosylated protein. After partial de-glycosylation, the sizes of the pro c-Met (175kDa) nd P125 c-Met (125kDa) are shifted to 160, 140kDa and 80kDa (PMID:23060267, PMID: 18187039).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254252).

  • Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free (ab254474)
    Anti-Met (c-Met) antibody [EPR19554-110] - BSA and Azide free (ab254474)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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