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Epigenetics and Nuclear Signaling Histones H3 Citrullated

Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab237370)

Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab237370)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR20358-13] to Histone H3 (citrulline R8)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

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Overview

  • Product name

    Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13]
    See all Histone H3 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR20358-13] to Histone H3 (citrulline R8)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK-293T cells transfected with a GFP-tagged PADI4 expression construct, then treated with 10mM CaCl2 for 2 hours and 10µM Ionomycin for 2 hours.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20358-13
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Citrullated

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab237370)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab237370)

    Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling Histone H3 with ab237370 at 1/100 dilution (red). Confocal image showing nuclear staining in HEK-293T cells transfected with a GFP-tagged PADI4 expression construct (green), then treated with 10mM CaCl2 for 2 hours and 10µM Ionomycin for 2 hours. Nuclear counterstain is DAPI (blue).

  • Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab237370)
    Alexa Fluor® 647 Anti-Histone H3 (citrulline R8) antibody [EPR20358-13] (ab237370)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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