Anti-Met (c-Met) antibody [CAL12] (ab237711)
![Anti-Met (c-Met) antibody [CAL12] (ab237711)](https://www.abcam.com/ps/products/237/ab237711/Images/ab237711-348927-anti-met-cmet-cal12-antibody-immunohistochemistry-human-lung-carcinoma-tissue.jpg "Anti-Met (c-Met) antibody [CAL12] (ab237711)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL12] to Met (c-Met)
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Met (c-Met) antibody [CAL12]
See all Met (c-Met) primary antibodies -
Description
Rabbit monoclonal [CAL12] to Met (c-Met) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human non-small cell lung carcinoma and lung carcinoma tissue. ICC: NCI-H1648 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity >99%. -
Clonality
Monoclonal -
Clone number
CAL12 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] (ab237711)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Met (c-Met) with ab237711 at 1/1000 dilution (0.45 μg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human lung carcinoma.
The section was incubated with ab237711 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] (ab237711)](https://www.abcam.com/ps/products/237/ab237711/Images/ab237711-315281-anti-met-c-met-antibody-cal12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] (ab237711)Formalin-fixed, paraffin-embedded human non-small cell lung carcinoma tissue stained for Met (c-Met) using ab237711 at 1 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] (ab237711)](https://www.abcam.com/ps/products/237/ab237711/Images/ab237711-315282-anti-met-c-met-antibody-cal12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] (ab237711)Formalin-fixed, paraffin-embedded human non-small cell lung carcinoma tissue stained for Met (c-Met) using ab237711 at 1 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
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![Anti-Met (c-Met) antibody [CAL12] (ab237711)](https://www.abcam.com/ps/products/237/ab237711/Images/ab237711-5-benefits-of-recombinant-antibodies.png)
Anti-Met (c-Met) antibody [CAL12] (ab237711)
