Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23000-42] to CD134 / OX40L receptor - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free
    See all CD134 / OX40L receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR23000-42] to CD134 / OX40L receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human PBMC treated with 10 µg/ml phytohaemagglutinin (PHA) for 2 days, whole cell lysate; human thymus and lymph node tissue lysates. IHC-P: Human thymus and Hodgkin's lymphoma tissue. IP: Human thymus tissue lysate. Flow cyt: Human peripheral blood mononuclear cell (PBMC) treated with 10 µg/ml phytohemagglutinin (PHA) for 2days. ICC/IF: Human PBMC treated with phytohaemagglutinin (PHA) (10 µg/ml for 2 days).

  • General notes

    ab264470 is the carrier-free version of ab264466. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264470 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD134 / OX40L receptor with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin's lymphoma (PMID/8547142) Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264466).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD134 / OX40L with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on T cells of human thymus (PMID/8547142) Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264466).

  • Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
    Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)

    CD134 / OX40L receptor was immunoprecipitated from 0.35 mg Human thymus lysate with ab264466 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264466 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Human thymus lysate 10ug

    Lane 2: ab264466 IP in Human thymus lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab264466 in Human thymus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264466).

  • Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
    Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)

    Flow cytometric analysis of 2% Paraformaldehyde fixed, 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 10 μg/ml phytohemagglutinin (PHA) for 2days labelling CD132 / OX40L receptor with ab264466 at 1/500 dilution 9Right) (Red) compared with a Rabbit monoclonal IgG (ab172730) control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab264466 (Right). (PMID: 9108415,17609274).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264466).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
    Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs  labelling CD34 / OX40L with ab264466 at 1/50 (10 ug/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in human PBMC treated with phytohaemagglutinin (PHA) (10 μg/ml for 2 days) (PMID: 9108415). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264466).

  • Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)
    Anti-CD134 / OX40L receptor antibody [EPR23000-42] - BSA and Azide free (ab264470)

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