Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: CHO cells
Purity: >= 98% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: Functional Studies, SDS-PAGE

Product name

Recombinant human CD272/BTLA protein (Active)
See all CD272/BTLA proteins and peptides
Biological activity

Measured in a competitive binding assay.
Purity

>= 98 % SDS-PAGE.
Endotoxin level
Expression system

CHO cells
Accession

Q7Z6A9
Protein length

Protein fragment
Animal free

No
Nature

Recombinant
- Species
  
  Human
- Sequence
  
  KESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCV KLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTT LYVTDVKSASERPSKDEMASRPWLLYR
- Predicted molecular weight
  
  33 kDa
- Amino acids
  
  31 to 157
- Additional sequence information
  
  The extracellular domain is fused to the N erminus of the Fc region of Human IgG1. NP_861445.3

Our Abpromise guarantee covers the use of ab214139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

Functional Studies

SDS-PAGE
Form

Lyophilized
Additional notes

This product was previously labelled as CD272
Concentration information loading...

Stability and Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Constituent: 99% PBS

Lyophilized from 0.2µm-filtered solution.

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

Reconstitute with 100 µl sterile water. Add 1X PBS to the desired protein concentration.

Alternative names
- B and T lymphocyte associated protein
- B and T lymphocyte attenuator
- B and T lymphocyte associated
- B- and T-lymphocyte attenuator
- B- and T-lymphocyte-associated protein
- BTLA
- BTLA_HUMAN
- BTLA1
- CD272
- CD272 antigen
- FLJ16065
- MGC129743
see all
Function

Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications

Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
N-glycosylated.
Cellular localization

Membrane.
Target information above from: UniProt accession Q7Z6A9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols