Recombinant human CD272/BTLA protein (Fc Chimera Active) (ab219654)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris, Sodium chloride, L-Arginine
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Functional Studies - Recombinant human CD272/BTLA protein (Fc Chimera Active) (ab219654)
Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind ab219654 with a linear range of 0.02 -0.625 μg/mL.
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SDS-PAGE - Recombinant human CD272/BTLA protein (Fc Chimera Active) (ab219654)SDS-PAGE under reducing (R) conditions using ab219654. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%. The reduced protein migrates as 46-66 kDa in SDS-PAGE due to glycosylation
