Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4106] to CD38 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free
    See all CD38 primary antibodies

  • Description

    Rabbit monoclonal [EPR4106] to CD38 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Specificity

    Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji and Ramos cell lysates, Human lymph node lysate. IHC-P: Human endometrial adenocarcinoma, tonsil, lymph node, skeletal muscle and normal colon tissues. Flow Cyt: Raji cells.

  • General notes

    ab176886 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    This IHC data was generated using the same anti-CD38 antibody clone, EPR4106, in a different buffer formulation (cat# ab108403).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Flow Cytometry - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD38 with ab108403 at 1/900 dilution (1.03 µg/ml) (Red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

    This IHC data was generated using the same anti-CD38 antibody clone, EPR4106, in a different buffer formulation (cat# ab108403).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    This IHC data was generated using the same anti-CD38 antibody clone, EPR4106, in a different buffer formulation (cat# ab108403).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    OI-RD Scanning - Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108403).

  • Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)
    Anti-CD38 antibody [EPR4106] - Low endotoxin, Azide free (ab176886)

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