Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5361(2)] to CD3 epsilon
Suitable for: WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-CD3 epsilon antibody [EPR5361(2)]
See all CD3 epsilon primary antibodies
Description

Rabbit monoclonal [EPR5361(2)] to CD3 epsilon
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues in the extracellular domain of Human CD3 epsilon (UniProt ID: P07766).
Positive control
- Jurkat and Human thymus lysates, HuT78 cells IP: Hut-78 whole cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5361(2)
Isotype

IgG
Research areas

Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

All lanes : Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Human thymus cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa
Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

Purified ab133628 at 1/50 dilution (2µg) immunoprecipitating CD3 epsilon in Hut-78 whole cell lysate.
Lane 1 (input): Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab133628 + Hut-78 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133628 in Hut-78 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa
Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

Flow cytometrical analysis of CD3 epsilon in HuT78 cells stained with ab133628 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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